Me yasa cryovials ke fashewa?Yadda za a kauce masa?

Me yasa cryovials ke fashewa?Yadda za a kauce masa?

Yayin gwajin, muna iya amfani da sucryovialsdon daskare samfurori, amma lokacin daskarewa tare da nitrogen na ruwa,cryovialssau da yawa fashewa, wanda ba wai kawai ya haifar da asarar samfurori na gwaji ba, amma yana iya haifar da lalacewa ga samfurori.Gwaji na haifar da lahani, to ta yaya za a hana hakan faruwa?

Dalilan:

Na farko,cryovialsba za a iya sanya shi kai tsaye a cikin lokacin ruwa na nitrogen na ruwa don adanawa ba.Domin abu na tube jiki da hula na kowacryovialssun bambanta, ƙimar haɓakawar thermal da ƙanƙancewa yayin daskarewa suma sun bambanta.Idan kun sakacryovialkai tsaye zuwa cikin lokacin ruwa, zaku iya barin nitrogen ruwa ya kwarara cikin bututu.Lokacin tayar da samfurin lokaci na gaba, sanyacryogenic-kwayoyina cikin wankan ruwa a 37°C, ruwan nitrogen da ke cikin bututun ya yi saurin tururi ya kuma fadada, amma iskar ba ta iya tserewa daga bututun cikin lokaci, wanda ya sa bututun cryopreservation ya fashe.

Yadda ake gujewa:

1. Kada a adanacryovialskai tsaye a cikin ruwa lokaci, amma a cikin gas lokaci.Ko kawai daskare shi kai tsaye a cikin firiji.Ka tuna kada a sanya shi kai tsaye a ƙasa da saman nitrogen na ruwa.

2. Yi amfani da juyawa na cikicryotubes.

Tabbas, har ma a cikin juyayicryotubesba za a iya sanya shi kai tsaye cikin yanayin ruwa ba, amma a jujjuyawar cikicryotubessuna da mafi kyawun juriya mai ƙarancin zafi fiye da iyakoki masu juyawa na waje, waɗanda zasu iya rage yiwuwar fashewa kuma sun fi aminci.Jujjuyawar wajecryotubehakika an tsara shi don daskarewa na inji, don haka bai dace da ajiyar ruwa na nitrogen ba.

3. Don haka menene ya kamata ku yi idan da gaske kuna buƙatar adana shi a cikin lokaci na ruwa?Dangane da wannan matsalar, a zahiri akwai hannun riga na cryopreservation bututu da aka kera musamman don wannan dalili, waɗanda za a iya amfani da su don rufe bututun cryopreservation sannan a sanya shi cikin yanayin ruwa.Tabbas, zaku iya amfani da fim ɗin rufewa, tef ɗin likitanci, da sauransu don rufe shi, ta yadda ba za a sami fashewa ba.