Mataki na farko zuwa gwajin ELISA mai nasara — zabar farantin ELISA daidai

TheELISAfarantin kayan aiki ne mai mahimmanci ga ELISA, gwajin immunosorbent mai alaƙa da enzyme.Akwai dalilai da yawa waɗanda ke shafar nasarar gwajin ELISA.Zaɓin kayan aiki mai kyau shine mataki na farko.Zaɓin microplate mai dacewa zai taimaka gwajin ya yi nasara.

Kayan abu naELISAFarantin gabaɗaya polystyrene ne (PS), kuma polystyrene yana da ƙarancin kwanciyar hankali na sinadarai kuma ana iya narkar da shi ta hanyar nau'ikan kaushi iri-iri (kamar hydrocarbons aromatic, halogenated hydrocarbons, da sauransu), kuma ana iya lalata shi da ƙarfi acid da alkalis.Ba mai juriya ga maiko ba kuma cikin sauƙin canza launin bayan an fallasa shi zuwa hasken UV.

Wani iriELISAfaranti akwai?

✦Zaɓa da launi

Farantin haske:dace da ƙididdigewa da ƙima mai ƙarfi-lokaci immunoassays da ɗaurin kima;

Farin faranti:dace da kai-luminescence da chemiluminescence;

Baki farantin:dace da fluorescent immunoassays da kuma dauri assays.

✦Zaɓa ta hanyar ɗaure ƙarfi

Farantin mai ƙarancin ɗauri:Ƙunƙasa yana ɗaure ga sunadaran ta hanyar haɗin hydrophobic na saman.Ya dace a matsayin mai ɗaukar lokaci mai ƙarfi don sunadaran macromolecular tare da nauyin kwayoyin halitta> 20kD.Matsakaicin haɗin furotin shine 200 ~ 300ng IgG/cm2.

Babban abin dauri:Bayan jiyya na saman, ƙarfin haɗin furotin yana haɓaka sosai, yana kaiwa 300 ~ 400ng IgG/cm2, kuma nauyin kwayoyin halitta na babban furotin mai ɗaure shine> 10kD.

✦Kira ta siffar kasa

Lebur kasa:ƙananan ƙididdiga masu mahimmanci, dacewa don ganowa tare da masu karanta microplate;

U kasa:Ƙididdigar refractive yana da girma, wanda ya dace don ƙarawa, haɓakawa, haɗuwa da sauran ayyuka.Kuna iya lura da canje-canjen launi kai tsaye ta duban gani ba tare da sanya shi akan mai karanta microplate ba don tantance ko akwai daidaitaccen maganin rigakafi.