Za a iya raba faranti na al'adar tantanin halitta zuwa ƙasa mai lebur da zagaye ƙasa (U-dimbin yawa da V-dimbin yawa) bisa ga siffar ƙasa;Yawan ramukan al'ada shine 6, 12, 24, 48, 96, 384, 1536, da dai sauransu;Dangane da kayan daban-daban, akwai farantin Terasaki da farantin al'adun sel na yau da kullun.Zaɓin takamaiman ya dogara da nau'in sel masu al'ada, ƙarar al'adun da ake buƙata da dalilai na gwaji daban-daban.
(1) Bambanci da zaɓi na lebur da zagaye ƙasa (U-dimbin yawa da V) faranti na al'ada
Siffofin daban-daban na faranti na al'ada suna da amfani daban-daban.Kwayoyin al'ada galibi suna ƙasa lebur, wanda ya dace da kallon gani a gani, tare da bayyanannen yanki na ƙasa da daidaitaccen matakin ruwa na al'adar tantanin halitta.Sabili da haka, lokacin yin MTT da sauran gwaje-gwaje, ana amfani da farantin ƙasa mai lebur gabaɗaya, ba tare da la'akari da ko an haɗa sel zuwa bango ko dakatar da su ba.Dole ne a yi amfani da farantin al'adun ƙasa mai lebur don auna ƙimar abin sha.Kula da hankali na musamman ga kayan, kuma yi alama "Al'adun Nama (TC) da aka bi da su" don al'adun tantanin halitta.
Ana amfani da faranti U-dimbin yawa ko V a gabaɗaya a wasu buƙatu na musamman.Alal misali, a cikin ilimin rigakafi, lokacin da aka haɗu da lymphocytes daban-daban guda biyu don al'ada, suna buƙatar tuntuɓar juna da ƙarfafa juna.A wannan lokacin, ana amfani da faranti U-dimbin yawa saboda sel za su taru a cikin ƙaramin yanki saboda tasirin nauyi.Hakanan za'a iya amfani da farantin al'adar zagaye na ƙasa don gwajin haɗakar isotope, wanda ke buƙatar kayan tattara tantanin halitta don tattara al'adun tantanin halitta, kamar "al'adun lymphocyte gauraye".Ana amfani da faranti mai siffar V sau da yawa don kashe sel da gwaje-gwajen agglutination na jini na rigakafi.Hakanan ana iya maye gurbin gwajin kashe tantanin halitta da farantin U-dimbin yawa (bayan ƙara sel, centrifuge a ƙananan gudu).
(2) Bambance-bambance tsakanin farantin Terasaki da farantin al'adun cell na yau da kullun
An fi amfani da farantin Terasaki don bincike na crystallographic.Zane samfurin ya dace don kallon crystal da nazarin tsarin.Akwai hanyoyi guda biyu: zama da rataye digo.Hanyoyi guda biyu suna amfani da saitin samfur daban-daban.An zaɓi polymer class ɗin kristal azaman kayan, kuma kayan na musamman sun dace don lura da tsarin crystal.
Farantin al'adar tantanin halitta an yi shi ne da kayan PS, kuma kayan ana bi da su a saman, wanda ya dace da haɓakar tantanin halitta da haɓakawa.Tabbas, akwai kuma kayan haɓakar ƙwayoyin sel na planktonic, kazalika da ƙarancin ɗauri.
(3) Bambance-bambance tsakanin farantin al'adun tantanin halitta da farantin Elisa
Farantin Elisa gabaɗaya ya fi tsada fiye da farantin al'adun tantanin halitta.An fi amfani da farantin tantanin halitta don al'adun tantanin halitta kuma ana iya amfani da su don auna ma'aunin furotin;Farantin Elisa ya haɗa da farantin rufi da farantin amsawa, kuma gabaɗaya baya buƙatar amfani da al'adun tantanin halitta.Ana amfani da shi musamman don gano furotin bayan amsawar da ke da alaƙa da enzyme na rigakafi, yana buƙatar buƙatu mafi girma da takamaiman alamar aikin enzyme.
(4) Yankin ƙasan rami da shawarar adadin ruwa na faranti daban-daban da aka saba amfani da su
Matsayin ruwa na al'adar da aka ƙara zuwa faranti daban-daban bai kamata ya yi zurfi sosai ba, gabaɗaya tsakanin kewayon 2 ~ 3mm.Ana iya ƙididdige adadin ruwan da ya dace na kowane rami na al'ada ta hanyar haɗa yankin ƙasa na ramuka daban-daban.Idan an ƙara yawan ruwa mai yawa, musayar iskar gas (oxygen) za ta yi tasiri, kuma yana da sauƙin zubarwa yayin tafiyar motsi, yana haifar da gurɓatacce.Ƙayyadadden ƙwayar tantanin halitta ya dogara da manufar gwaji.
Lokacin aikawa: Nuwamba-04-2022