Samfurin tarin, ajiya da buƙatun sufuri don gwaje-gwaje na gama gari

Samfurin tarin, ajiya da buƙatun sufuri don gwaje-gwaje na gama gari

1. Tari da adana samfuran cututtukan cututtuka:

☛ Sashin daskararre: Cire tubalan nama masu dacewa kuma adana su a cikin ruwa nitrogen;

☛Yankin paraffin: Cire tubalan nama masu dacewa kuma adana su a cikin 4% paraformaldehyde;

☛ faifan salula: An gyara faifan tantanin halitta a cikin 4% paraformaldehyde na tsawon mintuna 30, sannan a maye gurbinsu da PBS kuma a nutsar da su cikin PBS kuma a adana su a 4°C.

2. Tattara da adana samfuran ilimin halitta:

☛ Fresh tissue: Yanke samfurin a ajiye shi cikin ruwa nitrogen ko -80°C firiji;

☛ Samfuran Paraffin: Ajiye a zafin jiki;

☛ Cikakken samfurin jini: Ɗauki adadin jinin da ya dace kuma a ƙara EDTA ko heparin anticoagulation tube tarin jini;

☛ Samfurori na ruwa na jiki: centrifugation mai sauri don tattara laka;

☛ Samfuran Kwayoyin: Ana shafa ƙwayoyin sel da TRIzol kuma ana adana su a cikin ruwa nitrogen ko -80°C firiji.

3. Tari da adana samfuran gwajin furotin:

☛ Fresh tissue: Yanke samfurin a ajiye shi cikin ruwa nitrogen ko -80°C firiji;

☛ Cikakken samfurin jini: Ɗauki adadin jinin da ya dace kuma a ƙara EDTA ko heparin anticoagulation tube tarin jini;

☛Specimens cell: Kwayoyin suna cike da ruwa tare da maganin cell lysis sannan a adana su a cikin ruwa nitrogen ko -80°C firiji.

4. Tari da ajiya na ELISA, radioimmunoassay, da samfuran gwaji na biochemical:

☛Samfurin jini (plasma): Ɗaukar jini gaba ɗaya a zuba a cikin bututu mai hana ruwa gudu (anticoagulation tube), centrifuge a 2500 rpm na kimanin minti 20, a tattara abin da ke sama, sannan a adana shi a cikin ruwa nitrogen ko a cikin firiji -80 ° C;

☛Samfurin fitsari: sai a saka samfarin a 2500 rpm na kimanin mintuna 20, sannan a ajiye shi a cikin firiji na nitrogen ko -80°C;koma zuwa wannan hanyar don ruwa na thoracic da ascites, ruwa na cerebrospinal, da ruwan lavage na alveolar;

☛ Samfuran Kwayoyin: Lokacin gano abubuwan da aka ɓoye, sanya samfuran a 2500 rpm na kusan mintuna 20 kuma adana su cikin ruwa nitrogen ko -80 ° C firiji;lokacin gano abubuwan da ke cikin salula, Rarraba dakatarwar tantanin halitta tare da PBS kuma daskare kuma a narke akai-akai don lalata ƙwayoyin sel da sakin abubuwan haɗin ciki.Centrifuge a 2500 rpm na kimanin minti 20 kuma tattara mai girma kamar yadda yake sama;

☛Samfurin nama: Bayan an yanke samfuran, a auna su a daskare su a cikin ruwa na nitrogen ko -80 ° C don amfani daga baya.

5. Tarin samfurori na metabolomics:

☛Samfurin fitsari: Sanya samfurin a 2500 rpm na kimanin minti 20 kuma adana shi a cikin ruwa nitrogen ko -80 ° C firiji;koma zuwa wannan hanyar don maganin thoracic da ascites, ruwa na cerebrospinal, ruwan lavage na alveolar, da dai sauransu;

☛Bayan yanke samfurin nama, a auna shi a daskare shi a cikin ruwa nitrogen ko firiji -80 ° C don amfani daga baya;