Yadda ake amfani da bututun cryopreservation a kimiyyance kuma daidai
Yin amfani da bututun cryopreservation kimiyya ne, ba abu ne mai sauƙi ba kamar buɗe tankin nitrogen na ruwa, sanya shi cikin bututun cryopreservation da rufe tankin nitrogen na ruwa.Yin amfani da kimiyya da daidaitaccen bututun cryopreservation na iya guje wa asarar samfuran da kuma kare amincin masu gwaji.
Yin amfani da bututun cryopreservation kimiyya ne, ba abu ne mai sauƙi ba kamar buɗe tankin nitrogen na ruwa, sanya shi cikin bututun cryopreservation da rufe tankin nitrogen na ruwa.Yin amfani da kimiyya da daidaitaccen bututun cryopreservation na iya guje wa asarar samfuran da kuma kare amincin masu gwaji.
Bututu mai daskarewa: matakan daskarewa
A wanke kwayoyin halitta tare da maganin PBS da aka rigaya, a tsotse maganin, kuma a rufe sel tare da maganin da ke dauke da trypsin da EDTA (mai bakin ciki Layer ya isa, kuma ana buƙatar ƙaddarawa na trypsin da EDTA bisa ga layin salula).
Sanya sel a 37 ℃ na mintuna 3-5.
Bayan sel sun rabu daga ƙasa, an gama shiryawa, ana ƙara matsakaicin da ke ɗauke da jini, kuma ana dakatar da sel a hankali tare da pipette.
Centrifuge dakatarwar tantanin halitta (500 xg, 5 min) da sake dawowa tare da matsakaicin mai ɗauke da jini.
Ƙididdiga ta salula.
Centrifuge dakatarwar tantanin halitta (500 xg, mintuna 5), cire maɗaukakin abu, da sake dawo da sel tare da matsakaici mai ɗauke da ruwan magani na ƙarar da ta dace.
Mix sel da maganin cryopreservation (60% matsakaici, 20% fetal bovine serum, 20% DMSO) a cikin 1: 1 girma rabo, sa'an nan kuma canja wurin su zuwa Cryo STM cryopreservation tube.Yawan sel daskararre shine 1-5 × 106 guda/ml.
Cryo mai dauke da sel STM cryopreservation tube ana bada shawarar don kwantar da hankali a cikin adadin - 1 K/min, kuma ana iya sanya bututun cryopreservation a cikin akwati mai dauke da isopropanol a -70 ℃.Idan Cryo STM cryopreservation tube Stores sauran samfurori, wanda za a iya kai tsaye sanya a -20 ℃, - 70 ℃ ko gas lokaci na ruwa nitrogen.Don tabbatar da cewa samfurin yana daskarewa daidai, 4 ml da 5 ml Cryo Ana buƙatar bututun cryopreservation na sTM a cikin firiji a -20 ℃ na dare, sannan a tura shi zuwa lokacin iskar gas na - 70 ℃ ko nitrogen na ruwa.
Sannan canja wurin bututun cryopreservation na Cryo.sTM zuwa tankin ruwa na nitrogen.Domin kauce wa gurbacewa (kamar mycoplasma) da la'akari aminci, don Allah sanya Cryo.sTM cryopreservation tube a cikin iskar gas lokaci na ruwa nitrogen, ba a cikin ruwa lokaci.
Yadda ake amfani da bututun cryopreservation a kimiyyance kuma daidai?Kamfaninmu ya ƙunshi ƙwararrun ƙwararrun masana'antu da ƙwarewar kasuwa don samar da samfuran don filin binciken kimiyyar rayuwa da sabis don masu binciken kimiyya.Ba wai kawai zai iya biyan buƙatun musamman na abokan ciniki na R & D akan nau'ikan samfura da marufi ba, amma kuma ya dace da cikakkiyar buƙatun masana'antun samarwa a kowane matakai daga ƙaramin sikelin, matsakaicin matsakaici zuwa manyan samarwa.
Lokacin aikawa: Nuwamba-25-2022